18008 Pip5k1a Status: Cancelled
| Gene | Pip5k1a |
| Chromosome | 3 |
| Deletion Start | 95,106,448 |
| Deletion End | 95,071,894 |
| Deletion Size | 34,555 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,105,612 | 95,105,633 | GCACTTCTTATGCCTTCCTGAC |
| TUP | 95,105,581 | 95,105,608 | CCTTAGTGCTGGGATCATGGGAGCTTAC |
| TUR | 95,105,555 | 95,105,576 | CAATGTTTCCCTCTGTGCTGAC |
| TDF | 95,072,501 | 95,072,519 | GAAGGCAGCAACCTGACAC |
| TDP | 95,072,471 | 95,072,500 | CGGCTCACCATTACAATGACTTTCGATTCA |
| TDR | 95,072,448 | 95,072,466 | GAAGGCAACAGGCGCATAG |
PCR Primers
| Primer | Sequence |
| SU | AAGGTAGCTTTGGCGGAGAG |
| SD | GCAGGAAGACCCAGTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
