Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

18011 Mrps21 Status: ES cell colonies screened / QC positives

Gene	Mrps21
Chromosome	3
Deletion Start	95,870,573
Deletion End	95,862,745
Deletion Size	7,829
Genome Build	38

MGI ID	MGI:1913542
NCBI Gene ID	66292
BAC ID	220H12
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 3

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	95,870,450	95,870,468	ACCAGCCGTGGGAAATGTG
TUP	95,870,425	95,870,446	TGATCCTGCACCCAGCCTACCA
TUR	95,870,395	95,870,413	GAGCTGGCACCAGTGGTAG
TDF	95,863,688	95,863,708	TGTGGGACATGGGAAATGGTG
TDP	95,863,660	95,863,685	TGAGGCTGTTGCAATGCTCTCCTTTG
TDR	95,863,631	95,863,651	AAACACAGGCCCTCGTCATAG

PCR Primers

Primer	Sequence
SU	CGTAGTGAAAGAAATGTTGCTAAC
SD	CTAGCAACAAATATTACTGCTCC
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	74 bp	+	+	-
TDF	TDR	78 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
18011A-A7	VGB6	C57BL/6NTac
18011A-A11	VGB6	C57BL/6NTac
18011A-C4	VGB6	C57BL/6NTac
18011A-G12	VGB6	C57BL/6NTac