| Gene | Bmx |
| Chromosome | X |
| Deletion Start | 164,249,147 |
| Deletion End | 164,218,335 |
| Deletion Size | 30,813 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 164,248,305 | 164,248,329 | GCCATAGAGCAACAGAATATGTGTC |
| TUP | 164,248,279 | 164,248,304 | TCCGTTGACTTCCACGTCCCTTAGTC |
| TUR | 164,248,251 | 164,248,275 | GTACAGCTAAAGATAACGTCTGGAG |
| TDF | 164,218,583 | 164,218,606 | GGAGAGGAAGAAAGGAACTGAGTG |
| TDP | 164,218,553 | 164,218,577 | AAGAAGACAACTTAGCTATGGGACC |
| TDR | 164,218,514 | 164,218,533 | TCAGCAAAGGGACAGGTCAC |
PCR Primers
| Primer | Sequence |
| SU | TCCTGGAGTGCAGTTTGAAAG |
| SD | CTTGGGCCTGACTCCAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18015A-H12 | VGB6 | C57BL/6NTac | |
| 18015B-F5 | VGB6 | C57BL/6NTac | |
