18019 Gle1 Status: Cancelled
| Gene | Gle1 |
| Chromosome | 2 |
| Deletion Start | 29,958,551 |
| Deletion End | 29,935,503 |
| Deletion Size | 23,049 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,935,540 | 29,935,557 | TCCGCAACACCAGCAAAG |
| TUP | 29,935,562 | 29,935,582 | CCTGCGCTACGACCGCGAGTG |
| TUR | 29,935,622 | 29,935,642 | TCACTAGGGAGCCTGCTAAAC |
| TDF | 29,958,450 | 29,958,471 | TGTCTGCAACGAAGGGAGATTC |
| TDP | 29,958,475 | 29,958,497 | TGCCCAGAGGCTTTCTGACAACC |
| TDR | 29,958,509 | 29,958,529 | TGTGACGGAGGACATCAGGAG |
PCR Primers
| Primer | Sequence |
| SU | TCTTATCCACCTCAAACCGC |
| SD | TGGAGGGCTCACTACAGGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
