Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

18021 Mapk11 Status: ES cell colonies screened / QC positives

Gene	Mapk11
Chromosome	15
Deletion Start	89,149,510
Deletion End	89,143,730
Deletion Size	5,781
Genome Build	38

MGI ID	MGI:1338024
NCBI Gene ID	19094
BAC ID	432M20
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 15

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	89,148,797	89,148,817	GGGCTTGGAGACCAGAGATTG
TUP	89,148,772	89,148,795	AGTCTCGATCGTGGGCAGCATCCA
TUR	89,148,726	89,148,744	CCCGTCAACAAGGGCTATC
TDF	89,144,230	89,144,248	TGGCCCACGCATACTTCAG
TDP	89,144,203	89,144,229	CCAGTACCATGACCCTGACGATGAGCC
TDR	89,144,165	89,144,186	CCTTGGCCTCAACACTTTCATC

PCR Primers

Primer	Sequence
SU	ACCACGTGCTCAAGCTCAC
SD	TTGAGCATCTCCAACCACTG
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	92 bp	+	+	-
TDF	TDR	84 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
18021A-F1	VGB6	C57BL/6NTac
18021A-F6	VGB6	C57BL/6NTac
18021A-F9	VGB6	C57BL/6NTac
18021A-F12	VGB6	C57BL/6NTac