| Gene | Pttg1 |
| Chromosome | 11 |
| Deletion Start | 43,425,667 |
| Deletion End | 43,420,329 |
| Deletion Size | 5,339 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,424,835 | 43,424,855 | TCGGCAAAGTGTTCAATGCTC |
| TUP | 43,424,814 | 43,424,834 | CAGCCGTGCCTAAAGCCAGCA |
| TUR | 43,424,778 | 43,424,798 | TTTCGGCAACTCTGTTGACTG |
| TDF | 43,420,892 | 43,420,912 | TGGTGATAGCAGTGGGTGAAG |
| TDP | 43,420,865 | 43,420,890 | TGAACACAGAGCTGCTTAGGCTAGGA |
| TDR | 43,420,828 | 43,420,851 | GAGTGCTCATCCAGTCTCAGTTAC |
PCR Primers
| Primer | Sequence |
| SU | TGGAGGTTGAAGCAGGGTC |
| SD | ATGAGCTGGGAGTGACTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18027B-A7 | VGB6 | C57BL/6NTac | |
| 18027B-C4 | VGB6 | C57BL/6NTac | |
| 18027B-C8 | VGB6 | C57BL/6NTac | |
| 18027B-C12 | VGB6 | C57BL/6NTac | |
| 18027B-H6 | VGB6 | C57BL/6NTac | |
