18057 Rpe65 Status: ES cell colonies screened / QC no positives
| Gene | Rpe65 |
| Chromosome | 3 |
| Deletion Start | 159,624,806 |
| Deletion End | 159,599,236 |
| Deletion Size | 25,571 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 159,600,152 | 159,600,171 | AAGGGCCTGAGCAACACCTC |
| TUP | 159,600,175 | 159,600,198 | AAGTCCTAAGTGGGCTGTGCTGCA |
| TUR | 159,600,199 | 159,600,219 | AAGGACTCCTTCCAGTTCACC |
| TDF | 159,624,691 | 159,624,712 | GCCTGCATATCTCCTGGTTCTG |
| TDP | 159,624,717 | 159,624,744 | CCAAAGACTTGAGTGAAATTGCCAGGGC |
| TDR | 159,624,754 | 159,624,777 | TGGAAGGTCACAGGGATATTAGTC |
PCR Primers
| Primer | Sequence |
| SU | TGAGGTCAGCTCAGGACTGC |
| SD | CCATTAGAAACATGTCTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
