| Gene | Ywhae |
| Chromosome | 11 |
| Deletion Start | 75,764,740 |
| Deletion End | 75,733,031 |
| Deletion Size | 31,710 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,733,685 | 75,733,704 | CTTCTGGTGAGGGTGGAGTG |
| TUP | 75,733,706 | 75,733,729 | TTGGAAATCACTGCAGAGCCTCCC |
| TUR | 75,733,760 | 75,733,782 | GGCCCAAAGGAAGAAATCCAATC |
| TDF | 75,764,072 | 75,764,094 | CTCCATAACCAAGGGCTTCACTC |
| TDP | 75,764,105 | 75,764,133 | AATCTGCTGTTTGGCCTAACTGGCTACCA |
| TDR | 75,764,153 | 75,764,177 | TGGTGTTTAGGAAGATGTGCTACTG |
PCR Primers
| Primer | Sequence |
| SU | GTTCCAGCCTTTTCTGGC |
| SD | AGTGTGGCCGGAGACAATAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18059A-A11 | VGB6 | C57BL/6NTac | |
| 18059A-E1 | VGB6 | C57BL/6NTac | |
| 18059A-H3 | VGB6 | C57BL/6NTac | |
| 18059A-D6 | VGB6 | C57BL/6NTac | |
| 18059A-B8 | VGB6 | C57BL/6NTac | |
