18060 Aifm1 Status: ES cell colonies screened / QC no positives
| Gene | Aifm1 |
| Chromosome | X |
| Deletion Start | 48,513,335 |
| Deletion End | 48,475,019 |
| Deletion Size | 38,317 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,513,044 | 48,513,065 | CCAAGCTGAGGAATAGGCATAC |
| TUP | 48,513,019 | 48,513,042 | TATGCCGGCCTGACCTTCAAGAGA |
| TUR | 48,512,972 | 48,512,991 | GCAAAGCCTCCCAGAAGAAG |
| TDF | 48,475,643 | 48,475,662 | GGAGGACTACGGCAAAGGTG |
| TDP | 48,475,610 | 48,475,639 | TCTTCTACCTCAGGGACAAAGTTGTGGTGG |
| TDR | 48,475,587 | 48,475,609 | AAAGACGTTCCATAGCACAATCC |
PCR Primers
| Primer | Sequence |
| SU | CTACAGGGCCGCTAACTCTG |
| SD | GCAATTAAATACTTCCTGTCTACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
