18062 Gabarap Status: Cancelled
| Gene | Gabarap |
| Chromosome | 11 |
| Deletion Start | 69,994,595 |
| Deletion End | 69,991,716 |
| Deletion Size | 2,880 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,992,420 | 69,992,441 | ACCTGGTGCCTTCTGATCTTAC |
| TUP | 69,992,451 | 69,992,479 | CTTGACCTGTGGTGCACGTTTCTTTCTGT |
| TUR | 69,992,492 | 69,992,510 | GCCTCCTCCCACAGAGTTG |
| TDF | 69,994,330 | 69,994,348 | CGGCCCTTTCCTGCATCTG |
| TDP | 69,994,354 | 69,994,381 | ACCAGGCAATGCTTAAAGTGGTTCTTGA |
| TDR | 69,994,390 | 69,994,412 | CTCAACCATTCACCACCAATACC |
PCR Primers
| Primer | Sequence |
| SU | TGGGTACCAGTTTCGTCCTC |
| SD | AGGAGGTGGAATGACACAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
