| Gene | Idh1 |
| Chromosome | 1 |
| Deletion Start | 65,175,349 |
| Deletion End | 65,159,436 |
| Deletion Size | 15,914 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 65,174,452 | 65,174,474 | GGGACAAAAGGTGACCTAAATGC |
| TUP | 65,174,425 | 65,174,451 | TGAGAACTCCTGATTGTCCTTCCCAGC |
| TUR | 65,174,399 | 65,174,421 | CTGACTCAGTGGACACAGAAGAG |
| TDF | 65,160,360 | 65,160,381 | CCCTCCCTAAAGTACCTACCAG |
| TDP | 65,160,338 | 65,160,359 | TGTCCCTGTGGTGCCAGGACTT |
| TDR | 65,160,319 | 65,160,337 | GGCTCCCAGGTCCTCAAAC |
PCR Primers
| Primer | Sequence |
| SU | AGGGTGTGTTTCAGGGTCAC |
| SD | CACACTCATGGACAACAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 63 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18067A-C1 | VGB6 | C57BL/6NTac | |
| 18067A-F2 | VGB6 | C57BL/6NTac | |
| 18067A-D2 | VGB6 | C57BL/6NTac | |
