18069 Sgcb Status: ES cell colonies screened / QC no positives
| Gene | Sgcb |
| Chromosome | 5 |
| Deletion Start | 73,647,710 |
| Deletion End | 73,635,415 |
| Deletion Size | 12,296 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,647,275 | 73,647,296 | CCAGGTCTTGCTTCTGCCATTC |
| TUP | 73,647,250 | 73,647,272 | CGCCCACTGTGGTTTCTCGGAGA |
| TUR | 73,647,210 | 73,647,230 | AACCCACTAGACACGGGTCAG |
| TDF | 73,635,554 | 73,635,578 | GATGGCACACTCTTCAAAGTACAAG |
| TDP | 73,635,529 | 73,635,550 | CAGGTCACAACATGGGCTGCCA |
| TDR | 73,635,482 | 73,635,500 | GCGGATGCACCGTCTAATG |
PCR Primers
| Primer | Sequence |
| SU | GTTGATATAAAGGTAAAGGCATG |
| SD | AAATGTAGCTTTGGGTTAATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
