18083 Jmjd5 Status: ES cell colonies screened / QC positives
| Gene | Jmjd5 |
| Chromosome | 7 |
| Deletion Start | 125,461,273 |
| Deletion End | 125,452,240 |
| Deletion Size | 9,034 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 125,452,735 | 125,452,755 | CACCACTGAAAGCTGAACCAC |
| TUP | 125,452,756 | 125,452,780 | CCAGCAGCCCAACCCATCTTCTAGA |
| TUR | 125,452,782 | 125,452,801 | CCTGGGTGAGGACTGGATTC |
| TDF | 125,460,940 | 125,460,958 | TGTATTCGCCGCAAGAGTC |
| TDP | 125,460,961 | 125,460,984 | AGGCAGTGTACCCTCACGAGACGC |
| TDR | 125,461,019 | 125,461,039 | CGCCATGTCACCCTGATTTCC |
PCR Primers
| Primer | Sequence |
| SU | CTGTGGACACACAAACCCTG |
| SD | GAGGCCTGGGTACACAGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
