| Gene | Serf1 |
| Chromosome | 13 |
| Deletion Start | 100,114,075 |
| Deletion End | 100,108,226 |
| Deletion Size | 5,850 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,108,452 | 100,108,472 | GACCAGTCCGCTGAGACGTAG |
| TUP | 100,108,477 | 100,108,497 | CGCCTGAGGTCTGGAAGCCCA |
| TUR | 100,108,543 | 100,108,563 | TTAAAGCCAGCAGAGGCTGAG |
| TDF | 100,113,201 | 100,113,222 | CCTGAAACTTCTGACCCTCCTG |
| TDP | 100,113,226 | 100,113,249 | CTCCCTCCCGAGAGCCTGGGTTAT |
| TDR | 100,113,257 | 100,113,277 | TGGTTGCTGAATGAGGTGGTG |
PCR Primers
| Primer | Sequence |
| SU | GTTATTCTGGGCGACTTTGG |
| SD | CAGGCATGAATTTCTGATTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 112 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18088A-E5 | VGB6 | C57BL/6NTac | |
| 18088A-F7 | VGB6 | C57BL/6NTac | |
| 18088A-C5 | VGB6 | C57BL/6NTac | |
| 18088A-D11 | VGB6 | C57BL/6NTac | |
| 18088A-G6 | VGB6 | C57BL/6NTac | |
| 18088A-E10 | VGB6 | C57BL/6NTac | |
