18101 Alms1 Status: Cancelled
| Gene | Alms1 |
| Chromosome | 6 |
| Deletion Start | 85,623,197 |
| Deletion End | 85,587,649 |
| Deletion Size | 35,549 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,588,186 | 85,588,206 | GCTGGCAGGTTCTTTGTAGTC |
| TUP | 85,588,209 | 85,588,235 | AGGTTCTTCCTGACATTGACAGCTCCG |
| TUR | 85,588,237 | 85,588,256 | CACTGAGAGGCGCAGGAAAC |
| TDF | 85,622,814 | 85,622,836 | AAAGCTGCCAGGTCTGTAATGAG |
| TDP | 85,622,840 | 85,622,864 | TGAACCTGAAGGCTGCAGTACAGGC |
| TDR | 85,622,931 | 85,622,950 | TCCCATGAGCAGCAACCATC |
PCR Primers
| Primer | Sequence |
| SU | AAATTTGTAACTTGCGAACGTG |
| SD | TGTGTCCCCAGACAAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 137 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
