18105 Ralgds Status: ES cell colonies screened / QC one positive
| Gene | Ralgds |
| Chromosome | 2 |
| Deletion Start | 28,552,346 |
| Deletion End | 28,513,546 |
| Deletion Size | 38,801 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,514,122 | 28,514,140 | GGCGGAGGTCCTTAGTGTG |
| TUP | 28,514,142 | 28,514,170 | AGGAGGCTGCTGAGGAGACATTAGTTTCC |
| TUR | 28,514,178 | 28,514,200 | CAAGGGCCAAGCATCCTAAAGTG |
| TDF | 28,551,812 | 28,551,832 | GAGCCTTGGTGAGTAGCAGTG |
| TDP | 28,551,834 | 28,551,860 | AGCATCATGGAAGGCAGTGTGGATTCC |
| TDR | 28,551,863 | 28,551,881 | TCCAGCCAGAGCAGGTTTC |
PCR Primers
| Primer | Sequence |
| SU | GTCTAATCGCCTGCGACC |
| SD | GTGGGAGGAAAGGCTAGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
