18108 Capn5 Status: ES cell colonies screened / QC no positives
| Gene | Capn5 |
| Chromosome | 7 |
| Deletion Start | 98,162,077 |
| Deletion End | 98,123,842 |
| Deletion Size | 38,236 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,161,834 | 98,161,853 | TGCCTGGACAGACCTCTAAG |
| TUP | 98,161,808 | 98,161,831 | ATGGAACCCTCATGGACCGTGGGC |
| TUR | 98,161,773 | 98,161,792 | TGACCGCAGGAAGGAAAGTG |
| TDF | 98,124,153 | 98,124,172 | TGGGCTTGCACTGAGTGAAG |
| TDP | 98,124,130 | 98,124,152 | AGGCTTCTAGGTCCGGCTGCCCT |
| TDR | 98,124,041 | 98,124,062 | TCGGTGATTCCAAACCTGAGAG |
PCR Primers
| Primer | Sequence |
| SU | GGCCTGGCTAAACAAGACAG |
| SD | CAAACAAGGAGAGGGAAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 132 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
