| Gene | Nacc2 |
| Chromosome | 2 |
| Deletion Start | 26,090,419 |
| Deletion End | 26,059,870 |
| Deletion Size | 30,550 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 26,089,945 | 26,089,964 | TGCTGCCTATGTCACGTCTC |
| TUP | 26,089,921 | 26,089,944 | CTTCTGTGCCCATCCCGCTTCTGA |
| TUR | 26,089,887 | 26,089,906 | GGCGGCATCTCCATAGCTTC |
| TDF | 26,060,222 | 26,060,241 | TGCTGAGCGACGAAGTGATG |
| TDP | 26,060,190 | 26,060,215 | CCATCGTGGCTCTGAGAACTGATGCG |
| TDR | 26,060,153 | 26,060,172 | AGGCAGGGTTGGCTGAAGTG |
PCR Primers
| Primer | Sequence |
| SU | TGAACGCAGTAGGTGACAGG |
| SD | CCCAGCTTCTGCTTTCAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18111A-H11 | VGB6 | C57BL/6NTac | |
| 18111A-D7 | VGB6 | C57BL/6NTac | |
| 18111A-F9 | VGB6 | C57BL/6NTac | |
| 18111A-A12 | VGB6 | C57BL/6NTac | |
| 18111A-H7 | VGB6 | C57BL/6NTac | |
