| Gene | Vill |
| Chromosome | 9 |
| Deletion Start | 119,071,268 |
| Deletion End | 119,057,792 |
| Deletion Size | 13,477 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,058,329 | 119,058,348 | GCTACCCAGGGAGGATTCAG |
| TUP | 119,058,349 | 119,058,375 | TGACCTGCACTACTGGATTGGAAAGGA |
| TUR | 119,058,406 | 119,058,426 | TGCAGACATTGCACAAAGGAG |
| TDF | 119,070,417 | 119,070,436 | ACAGGTGTGAGGCGTAGGAG |
| TDP | 119,070,439 | 119,070,460 | AGGGCCCAGACTCCCAGATCGA |
| TDR | 119,070,491 | 119,070,512 | ACCCACCCAGACCACATTGAAC |
PCR Primers
| Primer | Sequence |
| SU | CTGTGGCCAGTTTCTAACTCTG |
| SD | CAGCTCTAACACATCGCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18120A-A1 | VGB6 | C57BL/6NTac | |
| 18120A-H5 | VGB6 | C57BL/6NTac | |
| 18120A-F7 | VGB6 | C57BL/6NTac | |
| 18120A-F3 | VGB6 | C57BL/6NTac | |
