18124 Btk Status: ES cell colonies screened / QC no positives
| Gene | Btk |
| Chromosome | X |
| Deletion Start | 134,574,054 |
| Deletion End | 134,542,720 |
| Deletion Size | 31,335 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,573,374 | 134,573,397 | CCACAGAGGGTGATTAGATACCTG |
| TUP | 134,573,344 | 134,573,370 | AAACATCTGGTGGCTCGATCACTCGTA |
| TUR | 134,573,296 | 134,573,318 | ACTGCCTCTTCTCTGAAGGAAAG |
| TDF | 134,542,857 | 134,542,875 | TACCACCCAGGGTTCCTTG |
| TDP | 134,542,835 | 134,542,856 | TGGATTCTCGATTCTACACTGT |
| TDR | 134,542,728 | 134,542,750 | CAGCCAGCTCAGGATTCTTCATC |
PCR Primers
| Primer | Sequence |
| SU | CTCACCATGTAGAATCAATCCC |
| SD | TTTAATGGCAACATTCCTACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 148 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
