| Gene | Pfn1 |
| Chromosome | 11 |
| Deletion Start | 70,654,477 |
| Deletion End | 70,652,032 |
| Deletion Size | 2,446 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,653,792 | 70,653,812 | AGTCGGCACGTTAGCCTCAAC |
| TUP | 70,653,769 | 70,653,791 | ATTCCTGGGAGGCCGATTCCGGA |
| TUR | 70,653,667 | 70,653,689 | CAGCAGCAACCTTATTCCTTCAC |
| TDF | 70,652,709 | 70,652,731 | CCTCAGTGTCAAGGCTGCATAAC |
| TDP | 70,652,677 | 70,652,703 | TTACTCTGCTGGTTATGGGAACCGTGG |
| TDR | 70,652,649 | 70,652,670 | GAGCCAGAGTCAGAACCCAAAG |
PCR Primers
| Primer | Sequence |
| SU | TGCAAAAGTTCGCTATCTGG |
| SD | TGCCTACTTAGTTTAGTCCTTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 146 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18130A-A5 | VGB6 | C57BL/6NTac | |
| 18130A-H11 | VGB6 | C57BL/6NTac | |
| 18130A-C10 | VGB6 | C57BL/6NTac | |
| 18130A-D6 | VGB6 | C57BL/6NTac | |
| 18130A-B4 | VGB6 | C57BL/6NTac | |
