18139 Rpl13a Status: ES cell colonies screened / QC no positives
| Gene | Rpl13a |
| Chromosome | 7 |
| Deletion Start | 45,128,711 |
| Deletion End | 45,125,938 |
| Deletion Size | 2,774 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,128,052 | 45,128,076 | ACCCTCCAGTAACAGCAATAAACAG |
| TUP | 45,128,024 | 45,128,049 | TGGCTGTGGAATGGGCTTCTTCTGTA |
| TUR | 45,128,005 | 45,128,023 | TCTGCATCCCAACCCAAAC |
| TDF | 45,126,184 | 45,126,203 | GGTGGAAGTACCAGGCAGTG |
| TDP | 45,126,159 | 45,126,182 | CAGCCACTCTGGAGGAGAAACGGA |
| TDR | 45,126,099 | 45,126,118 | CAGTGTCCGTCCTCACCAAG |
PCR Primers
| Primer | Sequence |
| SU | ACTTGATGGGAGTAATGGCG |
| SD | TATCCCGTGAGCAAGGATTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
