18147   Acot8     Status: ES cell colonies screened / QC positives

GeneAcot8
Chromosome2
Deletion Start164,804,814
Deletion End164,792,840
Deletion Size11,975
Genome Build38
MGI IDMGI:2158201
NCBI Gene ID170789
BAC ID394N14
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF164,804,734164,804,753ACCTCCGTAGTGTGCTGGTC
TUP164,804,711164,804,733ACGAGCGTGCTCAACCTCGAGCC
TUR164,804,655164,804,673GGGTGCGAGCCTAGTTCAG
TDF164,793,099164,793,119GTTCCAGTGTGCGGTTGTATG
TDP164,793,072164,793,095ACAGCTCTGCCCTGTGTCAGATCC
TDR164,793,012164,793,035TGTAAGCAAGGAGAAGGTGAGATG

PCR Primers

PrimerSequence
SUGAGTGAGTGGCTTACAGGGC
SDCTCCTGTCTTAAGGCCATGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR99 bp++-
TDFTDR108 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
18147A-F5VGB6C57BL/6NTac
18147A-E6VGB6C57BL/6NTac
18147A-E12VGB6C57BL/6NTac
18147A-A9VGB6C57BL/6NTac