| Gene | Dnajc10 |
| Chromosome | 2 |
| Deletion Start | 80,352,921 |
| Deletion End | 80,317,399 |
| Deletion Size | 35,523 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,317,786 | 80,317,805 | ACAGCAGGCACACGACTCAG |
| TUP | 80,317,809 | 80,317,832 | TTCGATCCCCACCACCTGAAACCC |
| TUR | 80,317,848 | 80,317,869 | ACTTGGCTTCTCCTGGTTTTAC |
| TDF | 80,352,059 | 80,352,079 | CAGTCAGCATAGCACAGTCTC |
| TDP | 80,352,080 | 80,352,100 | TGCGGAGTCTCGGCCTTCCTA |
| TDR | 80,352,158 | 80,352,177 | CCCTTAGCCGTGGACAAATG |
PCR Primers
| Primer | Sequence |
| SU | ACAGGGATGTGACCTTCAG |
| SD | TCTGAAGGTAACAGAGCCACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 119 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18150A-E11 | VGB6 | C57BL/6NTac | |
| 18150A-A12 | VGB6 | C57BL/6NTac | |
| 18150A-F2 | VGB6 | C57BL/6NTac | |
| 18150A-C9 | VGB6 | C57BL/6NTac | |
