| Gene | Mcm10 |
| Chromosome | 2 |
| Deletion Start | 5,010,692 |
| Deletion End | 4,991,340 |
| Deletion Size | 19,353 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,010,589 | 5,010,607 | GGGAGCCAGGACTGCTTAG |
| TUP | 5,010,555 | 5,010,584 | TTACATCCAACTTACATGTCAGACCAGGGA |
| TUR | 5,010,533 | 5,010,553 | GAGTCAGACCACCCAGTTCAG |
| TDF | 4,991,671 | 4,991,692 | CCTGTTCCTTCAGGGTCTCATC |
| TDP | 4,991,642 | 4,991,670 | CCCAGTGGTTTAAGAACTCCCACAAGTCC |
| TDR | 4,991,586 | 4,991,605 | TTCCCAGGGCCATCTGTTAC |
PCR Primers
| Primer | Sequence |
| SU | TGTCTCTCCTGCTTCAATGG |
| SD | TCAACAGAAGGAAACCCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18153A-H12 | VGB6 | C57BL/6NTac | |
| 18153A-B2 | VGB6 | C57BL/6NTac | |
| 18153A-D1 | VGB6 | C57BL/6NTac | |
| 18153A-G9 | VGB6 | C57BL/6NTac | |
| 18153A-G1 | VGB6 | C57BL/6NTac | |
| 18153A-B1 | VGB6 | C57BL/6NTac | |
