| Gene | Thra |
| Chromosome | 11 |
| Deletion Start | 98,764,572 |
| Deletion End | 98,753,589 |
| Deletion Size | 10,984 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,753,767 | 98,753,787 | GCCTGCTGCTTACCTTAGTCC |
| TUP | 98,753,794 | 98,753,820 | TGAGGGCATGCTCGTATTTCTTAGGGA |
| TUR | 98,753,833 | 98,753,857 | CTCCTTCAAAGTGAACATCCCAATC |
| TDF | 98,764,295 | 98,764,315 | TGCGTGGACAAGATCGAGAAG |
| TDP | 98,764,316 | 98,764,338 | AGTCAGGAGGCCTACCTGCTGGC |
| TDR | 98,764,346 | 98,764,364 | TGCGGTGGTTGACGTAGTG |
PCR Primers
| Primer | Sequence |
| SU | TGTTATTCTGGTCCCGTTCC |
| SD | GGCCTGAAGGGAAATCTAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18156A-A1 | VGB6 | C57BL/6NTac | |
| 18156A-H11 | VGB6 | C57BL/6NTac | |
