18166 Zmat2 Status: ES cell colonies screened / QC no positives
| Gene | Zmat2 |
| Chromosome | 18 |
| Deletion Start | 36,798,063 |
| Deletion End | 36,793,945 |
| Deletion Size | 4,119 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,794,019 | 36,794,040 | GAGACAGGCTGTGAGATTTGAC |
| TUP | 36,794,041 | 36,794,064 | TGACTTCCTGACACCTCTTCGCGA |
| TUR | 36,794,066 | 36,794,085 | TCTCGAACCTGAACCCTGAG |
| TDF | 36,797,684 | 36,797,707 | TCACTGCCATTTAGAGTCTGTCTC |
| TDP | 36,797,717 | 36,797,741 | TGCATGCTGGGCAGTGCTTTATCAC |
| TDR | 36,797,779 | 36,797,803 | GCTTCAGGATTCTTTTCCGTCTTTC |
PCR Primers
| Primer | Sequence |
| SU | GTTGGTTAGATCATGGAGCAC |
| SD | GGGTATACCAGTACCCGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 120 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
