18196 Nt5c2 Status: ES cell colonies screened / QC one positive
| Gene | Nt5c2 |
| Chromosome | 19 |
| Deletion Start | 46,950,943 |
| Deletion End | 46,924,264 |
| Deletion Size | 26,680 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,950,066 | 46,950,085 | TCAACCCAGGTGTGTAGCAC |
| TUP | 46,950,033 | 46,950,060 | TGTAATCTCAGCACTTGGGAGGTAGGGC |
| TUR | 46,950,008 | 46,950,030 | ACAATGACTCAAATAGCCCAAGC |
| TDF | 46,924,599 | 46,924,619 | GCTGTTTGTCCAAGGCTTGAC |
| TDP | 46,924,566 | 46,924,595 | ACAGTGTGAGCACAGGTAATAATGAGCTGG |
| TDR | 46,924,516 | 46,924,537 | AAACATGCCATGCAGGTAAGTC |
PCR Primers
| Primer | Sequence |
| SU | GCATTTGGTTTATTTAGAATTGGC |
| SD | GCCAAAATGCCCAAGTATACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
