| Gene | Gm2a |
| Chromosome | 11 |
| Deletion Start | 55,109,577 |
| Deletion End | 55,098,167 |
| Deletion Size | 11,411 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,098,806 | 55,098,826 | CAGCATAGGGTGAGGGCTTTC |
| TUP | 55,098,828 | 55,098,852 | TGAACCGAGGGTTTGCAGAATGCCC |
| TUR | 55,098,889 | 55,098,909 | CCCTGCATCTGACCAAACAAG |
| TDF | 55,109,387 | 55,109,407 | TCACTACCCACGAGCAACTTC |
| TDP | 55,109,412 | 55,109,434 | TGCCTGACCTAGAGCTACCGAGC |
| TDR | 55,109,466 | 55,109,485 | CCCACCACTGCTCAAGATAC |
PCR Primers
| Primer | Sequence |
| SU | CTATCATCCTCACCCAACCG |
| SD | GAGGCTCAGGATGGAATGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18201A-A7 | VGB6 | C57BL/6NTac | |
| 18201A-G7 | VGB6 | C57BL/6NTac | |
