| Gene | Mat2b |
| Chromosome | 11 |
| Deletion Start | 40,692,537 |
| Deletion End | 40,679,923 |
| Deletion Size | 12,615 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 40,692,152 | 40,692,170 | ACTGTGCGGTGCTGTGAAC |
| TUP | 40,692,127 | 40,692,150 | CTCAGGCTCCCTGGTAGGCAGACG |
| TUR | 40,692,091 | 40,692,115 | TGCTCAAACCTTCCTGAATAAACTC |
| TDF | 40,680,408 | 40,680,427 | GCTCCAAAGCCTGACAACTG |
| TDP | 40,680,383 | 40,680,406 | CGCCATCTACATGGTGGCAGGTGA |
| TDR | 40,680,325 | 40,680,344 | GCCATTAAGGCAGCACACAG |
PCR Primers
| Primer | Sequence |
| SU | CTCCTAGGCATTTCGCAGTC |
| SD | ATCTGGGCTCAAGCAAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18203A-B11 | VGB6 | C57BL/6NTac | |
| 18203A-H6 | VGB6 | C57BL/6NTac | |
