| Gene | Tgif1 |
| Chromosome | 17 |
| Deletion Start | 70,850,884 |
| Deletion End | 70,844,549 |
| Deletion Size | 6,336 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,850,734 | 70,850,755 | GGATTGGTGGCTGTTCTCTTTG |
| TUP | 70,850,708 | 70,850,732 | TGAGGCTGTGTCTCTGATGGCAGTT |
| TUR | 70,850,612 | 70,850,631 | CGCTGAGCGTGGTTCTTGAC |
| TDF | 70,844,989 | 70,845,009 | CCCGTCAGTGATCTGCCATAC |
| TDP | 70,844,961 | 70,844,986 | CTGTGACTGCATTGAAGGATGGGCCT |
| TDR | 70,844,901 | 70,844,921 | GGGTGCTATTTGCGGTACATC |
PCR Primers
| Primer | Sequence |
| SU | CCTTCCCTCTGCTCTCTCAC |
| SD | CAACTAGATTCATACTTGCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 144 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18209C-D11 | VGB6 | C57BL/6NTac | |
| 18209C-F8 | VGB6 | C57BL/6NTac | |
