18210 Amhr2 Status: ES cell colonies screened / QC no positives
| Gene | Amhr2 |
| Chromosome | 15 |
| Deletion Start | 102,454,509 |
| Deletion End | 102,445,477 |
| Deletion Size | 9,033 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,445,611 | 102,445,630 | GCCAGTGGGTTGGATGAGTG |
| TUP | 102,445,634 | 102,445,657 | AGGAAAGGCAGAGCCACATGTCCC |
| TUR | 102,445,697 | 102,445,716 | GTACAGCAGAAGCACAGGAC |
| TDF | 102,453,819 | 102,453,843 | CCACTCTCCTACCTGAAATGTCTAC |
| TDP | 102,453,852 | 102,453,879 | TGTCTATTGGATTGGTCTGTGGAAGCCA |
| TDR | 102,453,893 | 102,453,913 | TCTTAGTCAAGCAGTGTGTCC |
PCR Primers
| Primer | Sequence |
| SU | GCACCTTCTTCCAAGGTCAG |
| SD | GACCTCCACAACCTCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
