18224   Rims4     Status: ES cell colonies screened / QC no positives

GeneRims4
Chromosome2
Deletion Start163,918,680
Deletion End163,879,097
Deletion Size39,584
Genome Build38
MGI IDMGI:2674366
NCBI Gene ID241770
BAC ID384H19
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF163,917,914163,917,935AGGAGGTGCAAGGCAGTGTAAG
TUP163,917,887163,917,913TGCTAACTACAGCAGTGCTACCCTTCA
TUR163,917,845163,917,864GCCACTCCAGGGACAAGAAC
TDF163,879,344163,879,362CCCGACTAGGGCTGATGTG
TDP163,879,319163,879,341ACTGCCCACAGCTGCTGAAGCAC
TDR163,879,288163,879,310GGAAGGGACAGAGAACGAAGTTG

PCR Primers

PrimerSequence
SUGAGCACCTCGCGTCTCGG
SDGAACCGTCACAAGCCCAAGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR91 bp++-
TDFTDR75 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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