18235 5830415F09Rik Status: Cancelled
| Gene | 5830415F09Rik |
| Chromosome | 4 |
| Deletion Start | 46,389,390 |
| Deletion End | 46,377,024 |
| Deletion Size | 12,367 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,389,239 | 46,389,256 | AAGAACGCGAGCCACCTG |
| TUP | 46,389,214 | 46,389,238 | ACGTACATTCTTGTCCTTGCCGCCA |
| TUR | 46,389,164 | 46,389,184 | CCGTTAGCCTTAGCATGTCAC |
| TDF | 46,377,467 | 46,377,485 | GCAGTTGCTCTGCTGTGTC |
| TDP | 46,377,439 | 46,377,466 | CAGAAGACGCTTGTATAGTGGTCCGTCA |
| TDR | 46,377,390 | 46,377,414 | GGTGCATGGATCATTACTAAGAAGG |
PCR Primers
| Primer | Sequence |
| SU | GGACATTCACATCCTTTCCC |
| SD | TCCCTTTCAAACCACAATGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
