| Gene | Katnb1 |
| Chromosome | 8 |
| Deletion Start | 95,098,827 |
| Deletion End | 95,082,857 |
| Deletion Size | 15,971 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,083,075 | 95,083,098 | TGCTTGGTACTTGCTCTGAGATTC |
| TUP | 95,083,102 | 95,083,129 | TGAGATCTGTCTGGTCAAGCTGAAGAGC |
| TUR | 95,083,133 | 95,083,150 | CAGCCTTCCTCGCAACAC |
| TDF | 95,098,162 | 95,098,184 | TGCAGAGCAAGTATGAGAGGTAG |
| TDP | 95,098,187 | 95,098,211 | AGTCCCTCCAAACCCTGCAGCTGTC |
| TDR | | | TGCACACACACACACACC |
PCR Primers
| Primer | Sequence |
| SU | CTTGATGACCCAGGGACTTG |
| SD | TGTGTCTGGGAGAGCCAGCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18238A-B9 | VGB6 | C57BL/6NTac | |
| 18238A-C1 | VGB6 | C57BL/6NTac | |
| 18238A-G11 | VGB6 | C57BL/6NTac | |
| 18238A-D2 | VGB6 | C57BL/6NTac | |
