18240 Nfasc Status: Cancelled
| Gene | Nfasc |
| Chromosome | 1 |
| Deletion Start | 132,642,765 |
| Deletion End | 132,611,532 |
| Deletion Size | 31,234 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 132,642,312 | 132,642,334 | GCTGTCCTCATTGTGTCTCTAAC |
| TUP | 132,642,286 | 132,642,310 | CAATACACCAGAGCTGATGCCCAGC |
| TUR | 132,642,243 | 132,642,267 | CTCTACCAGGCTATTCACCATCTTC |
| TDF | 132,611,659 | 132,611,676 | TAGCGGCACCACCCAATC |
| TDP | 132,611,634 | 132,611,658 | CCAACCGTGAGGTAGCTGGAGACAC |
| TDR | 132,611,598 | 132,611,616 | GCCCTGCTGCTGATCTGAG |
PCR Primers
| Primer | Sequence |
| SU | GAGTGCTCTACGCAAAGCTG |
| SD | CAGCTTCTGGAGCTGATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
