18241 Nsl1 Status: ES cell colonies screened / QC no positives
| Gene | Nsl1 |
| Chromosome | 1 |
| Deletion Start | 191,082,489 |
| Deletion End | 191,063,042 |
| Deletion Size | 19,448 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 191,063,338 | 191,063,358 | GCCTTCGCAGATGGAACGATG |
| TUP | 191,063,360 | 191,063,383 | CCAAGACTGGCTTGGGAAATGCCA |
| TUR | 191,063,427 | 191,063,447 | TTCGCCCTGTATGTTCCTGAC |
| TDF | 191,082,257 | 191,082,278 | GGAAGTCTTCTCCAGCCTCTAC |
| TDP | 191,082,279 | 191,082,305 | AGGAAAGCTGACTCCAAGCCTGACACA |
| TDR | 191,082,311 | 191,082,331 | GGTGTGGTTTCTACGTGTGTC |
PCR Primers
| Primer | Sequence |
| SU | GGAATGTCAGTTCCAGGACG |
| SD | GAACCACCTCAGAGCCTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
