| Gene | Scd2 |
| Chromosome | 19 |
| Deletion Start | 44,303,212 |
| Deletion End | 44,294,391 |
| Deletion Size | 8,822 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,294,483 | 44,294,504 | TGTTGAGGGTTGGAGAGAGTTC |
| TUP | 44,294,505 | 44,294,531 | AGAGCAGCTGGGAGCATTTAGCCATCA |
| TUR | 44,294,556 | 44,294,575 | CACCCGGTCCAGAAACAATC |
| TDF | 44,302,846 | 44,302,870 | GGTGTCTGTCTGTTCCGAGTAAATG |
| TDP | 44,302,875 | 44,302,901 | TGGGTTTAGCCATTCACTCACCTGCAG |
| TDR | 44,302,919 | 44,302,937 | TACCCAGTGGGCTCCTCAG |
PCR Primers
| Primer | Sequence |
| SU | ATTCTGACTCCTGGACACCG |
| SD | TTCATTCCATCTGGCAAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18247A-A4 | VGB6 | C57BL/6NTac | |
| 18247A-A7 | VGB6 | C57BL/6NTac | |
| 18247A-E2 | VGB6 | C57BL/6NTac | |
| 18247A-D4 | VGB6 | C57BL/6NTac | |
| 18247A-D8 | VGB6 | C57BL/6NTac | |
