| Gene | Apoh |
| Chromosome | 11 |
| Deletion Start | 108,414,365 |
| Deletion End | 108,395,347 |
| Deletion Size | 19,019 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,395,800 | 108,395,822 | GTTCTGTTGTGTTCAGTCTGTCC |
| TUP | 108,395,824 | 108,395,851 | AAGCCGGATGACCTACCATTTGCTACGG |
| TUR | 108,395,865 | 108,395,883 | CCCAGGGTCGTAGGATGTC |
| TDF | 108,413,471 | 108,413,491 | CTGCCCAAAGGATAGAAGAGC |
| TDP | 108,413,492 | 108,413,515 | TCAGGCTGCAGGGATGGATTCCTT |
| TDR | 108,413,532 | 108,413,550 | TTAGGCCCAGGTGCTTGTG |
PCR Primers
| Primer | Sequence |
| SU | CCAGACAGTGCCAGTAATCATC |
| SD | TGGCCATGATACCCAAACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18265A-C11 | VGB6 | C57BL/6NTac | |
| 18265A-H8 | VGB6 | C57BL/6NTac | |
