18274 Ptpn11 Status: ES cell colonies screened / QC no positives
| Gene | Ptpn11 |
| Chromosome | 5 |
| Deletion Start | 121,191,212 |
| Deletion End | 121,162,714 |
| Deletion Size | 28,499 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,190,729 | 121,190,747 | ATCTGGCCGTGGGATTGTC |
| TUP | 121,190,698 | 121,190,725 | TCTGTTTGACGAATGTGGAAACTGAGGC |
| TUR | 121,190,672 | 121,190,693 | TCCTGTGACGAGCACGTTATTC |
| TDF | 121,163,531 | 121,163,552 | CTGTATGCTCCTTTCGCACTTG |
| TDP | 121,163,500 | 121,163,527 | CATTTGCTTTACTGGGAGGCTCTCCTGC |
| TDR | 121,163,446 | 121,163,465 | GAGCCACACTTGGCCTTGAC |
PCR Primers
| Primer | Sequence |
| SU | TCAGAGGAGGAATCCCACAC |
| SD | CACACATGGCACTGAAGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
