18284   Pafah1b3     Status: ES cell colonies screened / QC positives

GenePafah1b3
Chromosome7
Deletion Start25,297,449
Deletion End25,295,026
Deletion Size2,424
Genome Build38
MGI IDMGI:108414
NCBI Gene ID18476
BAC ID66B11
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF25,297,18925,297,209GCGTGCCTTTGACTCTTTCTG
TUP25,297,16125,297,186TTTCTCTGCAGCACCATCGGTTTGTA
TUR25,297,12825,297,152GACTACTTCGGGTTCCTTGTCTTTG
TDF25,295,24325,295,262GACCCTGGGTTTGTGCATTC
TDP25,295,21325,295,242TGATGGCACCATAAGTCACCATGACATGTA
TDR25,295,19125,295,211GCGGCTCAGGTGTAGGTAATC

PCR Primers

PrimerSequence
SUTAGATTGTCCCTGGTCCCTG
SDCAGAGCACCAGGAAGTTAGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR82 bp++-
TDFTDR72 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
18284A-C5VGB6C57BL/6NTac
18284A-F12VGB6C57BL/6NTac
18284A-H4VGB6C57BL/6NTac
18284A-D3VGB6C57BL/6NTac