18291 Oprd1 Status: ES cell colonies screened / QC no positives
| Gene | Oprd1 |
| Chromosome | 4 |
| Deletion Start | 132,144,382 |
| Deletion End | 132,113,490 |
| Deletion Size | 30,893 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 132,144,169 | 132,144,189 | GCAACGTGCTCGTCATGTTTG |
| TUP | 132,144,146 | 132,144,167 | CATCGTCCGGTGAGTCAGCCGA |
| TUR | 132,144,042 | 132,144,062 | GCCTCTGTGCAGGACATTCAG |
| TDF | 132,113,779 | 132,113,796 | TGGACGCTGGTGGACATC |
| TDP | 132,113,756 | 132,113,778 | AATCGGCGCGACCCACTTGTGGT |
| TDR | 132,113,680 | 132,113,700 | GTCCAGGAAGGCGTAGAGAAC |
PCR Primers
| Primer | Sequence |
| SU | TTCTTCTGACTCCGAAGTGG |
| SD | CTTACTAATGGATACAGTTTAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 148 bp | + | + | - |
| TDF | TDR | 117 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
