18306 Bpifb3 Status: ES cell colonies screened / QC no positives
| Gene | Bpifb3 |
| Chromosome | 2 |
| Deletion Start | 153,932,817 |
| Deletion End | 153,919,636 |
| Deletion Size | 13,182 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 153,919,699 | 153,919,717 | GAGACAGTGGGCACGCTTG |
| TUP | 153,919,718 | 153,919,744 | CTCGGATTGACAAGGATGAACTGGGCA |
| TUR | 153,919,809 | 153,919,828 | AGCCTCAGCTCGAACATCTG |
| TDF | 153,932,066 | 153,932,088 | ACCCACCTCTGTGTCATGTCTAC |
| TDP | 153,932,095 | 153,932,122 | CAGAGAACCACAATCGTCAGAAACAGCA |
| TDR | 153,932,144 | 153,932,163 | CCACGGGTGAGGAAGGAAAG |
PCR Primers
| Primer | Sequence |
| SU | AAAGCATTTACCCTGGACCC |
| SD | GCACTCCAATAACTGAGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 130 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
