18322 Baiap2l2 Status: ES cell colonies screened / QC no positives
| Gene | Baiap2l2 |
| Chromosome | 15 |
| Deletion Start | 79,285,385 |
| Deletion End | 79,258,537 |
| Deletion Size | 26,849 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,284,614 | 79,284,632 | AATCCCGCCCTGGAGAATC |
| TUP | 79,284,590 | 79,284,613 | TGGTGTACCTGGGCAACAACTACC |
| TUR | 79,284,551 | 79,284,570 | GGCTGAGTACCAGCGTAGAC |
| TDF | 79,259,353 | 79,259,374 | CCACAGCCTTGATGACCTCTTG |
| TDP | 79,259,332 | 79,259,351 | ACCGGCCAGGCAATCCCACA |
| TDR | 79,259,295 | 79,259,313 | GCGAGCGGGACTGACTATC |
PCR Primers
| Primer | Sequence |
| SU | AGCTTACCTGCACTTGCCC |
| SD | GCACCTCCAAGACTTTGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
