18326 Myo7a Status: Cancelled
| Gene | Myo7a |
| Chromosome | 7 |
| Deletion Start | 98,117,730 |
| Deletion End | 98,083,512 |
| Deletion Size | 34,219 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,117,253 | 98,117,273 | GTCTCTGACCCTCAGCCCTTC |
| TUP | 98,117,228 | 98,117,252 | CCCGTGATCAGCCTGAGACTCATGA |
| TUR | 98,117,178 | 98,117,199 | ATCTGGCGCACTGCCTACATTC |
| TDF | 98,084,157 | 98,084,179 | GCTATCACAGTGGCTACCACAAG |
| TDP | 98,084,127 | 98,084,150 | CCTCTTCCTCAAGGCAGCACAGCC |
| TDR | 98,084,079 | 98,084,096 | GTGGCCTCCATGCACAAC |
PCR Primers
| Primer | Sequence |
| SU | CTGAACCAGGAGCAGAGTCC |
| SD | CCCTGGGCAGAAGACAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
