18329 Aldh7a1 Status: Cancelled
| Gene | Aldh7a1 |
| Chromosome | 18 |
| Deletion Start | 56,562,137 |
| Deletion End | 56,526,900 |
| Deletion Size | 35,238 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,561,758 | 56,561,780 | CAGCCTTGCTTGGGATCTGTAAC |
| TUP | 56,561,731 | 56,561,753 | TCGCCCTGGCTGAAATGTTGTGA |
| TUR | 56,561,680 | 56,561,703 | GCTAAGTCTGGGCAATGAATACTG |
| TDF | 56,527,012 | 56,527,035 | CTCCCATTCGTTTCCTTCTCTTCC |
| TDP | 56,526,985 | 56,527,011 | CCAGTACCATCAACTACAGCACGTCCC |
| TDR | 56,526,938 | 56,526,956 | CAAGCCCACGCAGTATCAC |
PCR Primers
| Primer | Sequence |
| SU | TGAGCGCTCGCAGAATGG |
| SD | GAGCACTGCAAATCATGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
