18373 Carkd Status: ES cell colonies screened / QC no positives
| Gene | Carkd |
| Chromosome | 8 |
| Deletion Start | 11,513,099 |
| Deletion End | 11,497,656 |
| Deletion Size | 15,444 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GCGCACTGAGGTTTCATTAC |
| TUP | | | TGACTTGCTAAATGTCTGGCAAAGACCTGG |
| TUR | | | GAAAGGTCTAAAGCCCAGAACTC |
| TDF | | | CACTGGTGCAGACTGGGTATC |
| TDP | | | TCAGGTGGCCTTGACCTTCACTCA |
| TDR | | | GGGCCACAGGACAAACTAGAAC |
PCR Primers
| Primer | Sequence |
| SU | GCGGAGGGTAATTGATGGAC |
| SD | GTGAGGGGTGACTAGTAACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
