18408 Rpain Status: ES cell colonies screened / QC one positive
| Gene | Rpain |
| Chromosome | 11 |
| Deletion Start | 70,977,684 |
| Deletion End | 70,970,458 |
| Deletion Size | 7,227 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CATCAGACACGGTTAGGAGAGTTG |
| TUP | | | TAAGCTCCGCCTTCTTCCGAACTCA |
| TUR | | | TGGGTGGTTCTTCTGGAATACAC |
| TDF | | | GGAGGCCGAAGTAGCATAATTTGAG |
| TDP | | | CCAGTAGTTCAAGACCAGCCTGAGCA |
| TDR | | | GGCAGTCATCTGCTGCTTTG |
PCR Primers
| Primer | Sequence |
| SU | CGAGAAGTTGGCCCAATAAG |
| SD | AAAGCTCCCTTGGCATCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
