18413 Arpc5 Status: Cancelled
| Gene | Arpc5 |
| Chromosome | 1 |
| Deletion Start | 152,774,424 |
| Deletion End | 152,766,728 |
| Deletion Size | 7,697 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CACCGCTGGTGTCCTAGTG |
| TUP | | | ACCTGAGCCAGTCTCTGCCCGGT |
| TUR | | | ACAGGAAAGCAAGACTCCAAGC |
| TDF | | | GTACCAGCCTTTCCCTGAGTG |
| TDP | | | CCCTCACACCTCCACACTGGCTTT |
| TDR | | | AGCCGGACAGCAAGAGATG |
PCR Primers
| Primer | Sequence |
| SU | TGCCAGCTTTCTGTCGAC |
| SD | TCACCCACTACTTGCCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
