18447 Reck Status: Cancelled
| Gene | Reck |
| Chromosome | 4 |
| Deletion Start | 43,895,882 |
| Deletion End | 43,875,609 |
| Deletion Size | 20,274 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GCTTGACCGCTTAGGATTGTC |
| TUP | | | TTCCTGCGCTTGCTCAGCGTTC |
| TUR | | | AAGCACGAGTTCGTGTCACATC |
| TDF | | | GCTCCATTTCTGGCACACTG |
| TDP | | | CACTGCTTGAAGTCCGAATCAGGAAGTC |
| TDR | | | CAATGAAACCCGCCCAAACTC |
PCR Primers
| Primer | Sequence |
| SU | ATAACAAAGAGCCAGGGTCC |
| SD | GCAAGCAGCAAGTGAGAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
